Chromatography

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with solutes) are: solids (adsorption), ionic groups on a resin (ion-exchange), liquids on an inert solid support (partitioning), and porous inert particles (size exclusion). The mixture to be separated is loaded onto the top of the column followed by more solvent. The different components in the sample mixture pass through the column at different rates due to differences in their partition behavior between the mobile phase and the stationary phase. The compounds are separated by collecting aliquots of the column effluent as a function of time.
Conventional Liquid is most commonly used in preparative scale work to purify and isolate some components of a mixture. It’s also used in ultra trace separations where small disposable columns are used once and then discarded. Analytical separations of solutions for detection or quantification typically use more sophisticated high-pressure liquid instruments.
In liquid , the separator is called the column and consists in most ...

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