How Does pH Buffer Affect Catalase’s Rate Of Reaction?

Factors that affect rate of reaction

Research Question:

How does pH buffer affect catalase's rate of reaction?


pH buffer:

a mixture of a weak acid and its conjugate base or a weak base and its conjugate acid. It is used to prevent changes in the pH of a solution. Buffer solutions are used as a means of keeping pH at a nearly constant value.

Hydrogen Peroxide: (H2O2)

Hydrogen peroxide is a clear colorless liquid, slightly more viscous than water. Due to its oxidizing properties, hydrogen peroxide is often used as a bleach or cleaning agent.


Catalase is an enzyme (catalyst) that catalyzes the decomposition of hydrogen peroxide to water and ...

Want to read the rest of this paper?
Join Essayworld today to view this entire essay
and over 50,000 other term papers

pH instability will cause the change in the bonds in the enzyme, which change the enzymes composition and therefore cause its denature. As enzyme was functioned by the lock and key theory, substrate (key) must binds with specific enzyme's activation site (lock), which has a specific shape. Therefore the denature of the enzyme will make the reaction lose an alternative pathway to form product with an lower activation energy and thus lower the rate of reaction.


Independent Variable:

pH of hydrogen peroxide (pH 3,5,7,10,13)

Dependent Variable:

Rate of reaction (amount of oxygen produced)

Reasons for Measuring

Ways to Measure

Because oxygen is the product of the reaction therefore measuring the change of oxygen concentration in air content could measure the rate of reaction.

Through oxygen gas sensor and gas chamber so that oxygen gas produced would not escape and can be sensed by the oxygen gas sensor.

Control ...

Get instant access to over 50,000 essays.
Write better papers. Get better grades.

Already a member? Login


How Does pH Buffer Affect Catalase’s Rate Of Reaction?. (2013, May 9). Retrieved March 26, 2019, from
"How Does pH Buffer Affect Catalase’s Rate Of Reaction?.", 9 May. 2013. Web. 26 Mar. 2019. <>
"How Does pH Buffer Affect Catalase’s Rate Of Reaction?." May 9, 2013. Accessed March 26, 2019.
"How Does pH Buffer Affect Catalase’s Rate Of Reaction?." May 9, 2013. Accessed March 26, 2019.
Join today and get instant access to this and 50,000+ other essays

Added: 5/9/2013 01:40:09 AM
Submitted By: s014213
Category: Biology
Type: Premium Paper
Words: 991
Pages: 4

Save | Report


Save and find your favorite essays easier

» How Does Light Affect The Rate ...
» Crystals: Does Surface Tension ...
» How Does Clinical Depression Af...
» Rates Of Reaction
» Chemistry Investigation
» How The Availability Of The Wea...
» Deregulation And How It Will Af...
» Growth Rate Of A Seed
» Excretion And Elimination Of To...
» The Application of Internationa...
Copyright | Cancel | Contact Us

Copyright © 2019 Essayworld. All rights reserved