Growth Dynamics Of E. Coli In Varying Concentrations Of Nutrient Broths, PH, And In The Presence Of An Antibiotic


Growth Dynamics of E. coli in Varying Concentrations of Nutrient Broths, pH, and
in the Presence of an Antibiotic
Department of Biology
Rensselaer Polytechnic Institute
Troy, NY 12180

Abstract

The purpose in this experiment of growth dynamics of E. coli in varying media
was to determine which media produces the maximum number of cells per unit time.
First a control was established for E. coli in a 1.0x nutrient broth. This was
used to compare the growth in the experimental media of 0.5x and 2.0x, nutrient
broths; nutrient broths with an additional 5.0mM of glucose and another with
5.0mM lactose; nutrient broths of varying pH levels: 6.0, 7.0, and 8.0; and
finally a nutrient broth in ...

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producing 89,3 01
cells/ml; glucose producing 411,951 cells/mL; lactose producing 477,441 cells/mL
and finally pH 6.0 producing 579,557cells/mL. The remaining four media, after
calculations, produced cell counts greater than the control: 2X with 1,087,009
cells/mL; 0.5X with 2,205,026 cells/mL; pH 8 with 3,583,750 cells/mL and finally
pH 7.0 with 8,090,325 cells/mL. From these results the conclusion can be made
that the environment is a controlling factor in the growth dynamics of E. coli.
This was found through the regulation of pH and nutrient concentrations. In the
presence of the drug/antibiotic, chloramphenicol, cell growth was minimal.

Introduction

E. coli grows and divides through asexual reproduction. Growth will continue
until all nutrients are depleted and the wastes rise to a toxic level. This is
demonstrated by the Log of the number of cells per unit volume versus Time
growth curve. This growth curve consists of four phases: Lag, Exponential,
Stationary, and ...

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The k value can be easily derived by knowing the number of cells in a
exponentially growing population at two different times. K is determined using
the equation k=(ln N-ln N0 )/t, where ln N is the natural log of the number of
cells at some time t, ln N0 is the natural log of the initial number of cells
and t is time. This equation allows one to calculate the numbers of cells in a
culture at any given time. The reciprocal of k is the mean doubling time, in
other words, the time required for the population to double, usually expressed
as cells per unit volume. (Edick 61-62)
Temperature is the most influential factor of growth in bacteria. The
optimal temperature of E. coli is 37C, ...

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Growth Dynamics Of E. Coli In Varying Concentrations Of Nutrient Broths, PH, And In The Presence Of An Antibiotic. (2008, April 26). Retrieved January 23, 2018, from http://www.essayworld.com/essays/Growth-Dynamics-E-Coli-Varying-Concentrations/82710
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"Growth Dynamics Of E. Coli In Varying Concentrations Of Nutrient Broths, PH, And In The Presence Of An Antibiotic." Essayworld.com. April 26, 2008. Accessed January 23, 2018. http://www.essayworld.com/essays/Growth-Dynamics-E-Coli-Varying-Concentrations/82710.
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PAPER DETAILS
Added: 4/26/2008 09:05:02 PM
Category: Science & Nature
Type: Premium Paper
Words: 2626
Pages: 10

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